Is also called the вЂspongeвЂ™ function, which can sequester small Variety of biological functions, such as signaling ( 9), acting as molecular decoys ( 10) and guiding ( 11). Long non-coding RNAs (lncRNAs), which are widelyДistributed in the human genome to modulate gene expression, have a Findings on theД«iological functions of OS may eventually be applied to the Promote the occurrence and development of OS. Microangiogenesis in promoting tumor development ( 4), imbalance of cell proliferation and Macromolecules and genes, including activated oncogenes ( 2), tumor suppressor gene inactivation Recently, the focus of research on OS has been on biological

The most common primary malignant bone tumor, arising frequently in Osteosarcoma (OS), originating from osteoblasts, is Taken together, the results of the present study suggest that lncRNA XLOC_005950 knockout may inhibit the progression of OS via hsaвЂ‘miRвЂ‘542вЂ‘3pвЂ‘mediated regulation of PFKM expression. Furthermore, lncRNA XLOC_005950, as the molecular sponge of miRвЂ‘542вЂ‘3p in OS, modulated the downstream target gene, PFKM. Overexpression of hsaвЂ‘miRвЂ‘542вЂ‘3p suppressed PFKM expression. Furthermore, lncRNA XLOC_005950 knockout upregulated hsaвЂ‘miRвЂ‘542вЂ‘3p expression and downregulated PFKM expression. Functional experiments indicated that lncRNA XLOC_005950 knockout decreased PFKM activity, the intracellular glucose and lactic acid content, and cell proliferation, while increasing apoptosis of OS cells. The results demonstrated that lncRNA XLOC_005950 expression was upregulated in OS tissues and cells. The dualвЂ‘luciferase reporter assay was performed to confirm the targeting associations between lncRNA XLOC_005950, hsaвЂ‘miRвЂ‘542вЂ‘3p and PFKM. Cell Counting KitвЂ‘8 assay, flow cytometry, PFKM activity, and glucose and lactic acid content determination were performed to assess the effects of lncRNA XLOC_005950 knockout and overexpression of hsaвЂ‘miRвЂ‘542вЂ‘3p on the phenotypes of OS cells.

CRISPR/Cas9 gene editing was used to knockout lncRNA XLOC_005950 expression in MG63 cells. lncRNA XLOC_005950, hsaвЂ‘miRвЂ‘542вЂ‘3p and PFKM expression in OS tissues and cells was detected via reverse transcriptionвЂ‘quantitative PCR analysis.

The present study aimed to investigate the involvement of the lncRNA XLOC_005950/hsaвЂ‘microRNA (miR)вЂ‘542вЂ‘3p/phosphofructokinase, muscle (PFKM) axis in the regulation of glucose metabolism, cell proliferation and apoptosis in the progression of OS. However, the effect of long nonвЂ‘coding RNAs (lncRNAs) on glycolysis in osteosarcoma (OS) cells remains unclear. Cancer cells use glucose via glycolysis to maintain tumor cell proliferation.

Removing xloc